This is a graph of the change in ACh release over 13 fifteen minute increments. For the first four collections, artificial cerebrospinal fluid is pumped through the microdialysis probes, to determine baseline ACh release. Beginning at the the fourth collection interval, D-CPP is infused into the nucleus accumbens, which almost immediately increases cortical ACh release in a dose-dependent fashion. The four lines represent: 1)Black = The control vehicle infusion of artifical CSF 2)Red = 10 uM D-CPP 3)Green = 100 uM D-CPP 4)Blue = 200 uM D-CPP. As displayed in the graph, the highest dose of D-CPP (200 uM) robustly increased ACh release in the PFC, while the two moderate doses produced a more moderate effect.
Intra-NAC administration of D-CPP increased ACh release in mPFC in a dose dependent fashion up to 400%, evidence of a significant Glutaminergic tone in the NAC of awake animals. This finding is interesting because NMDA, a ligand with opposite pharmacological properties also increases ACh release in mPFC.